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KMID : 0903619950360060829
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Journal of the Korean Society for Horticultural Science
1995 Volume.36 No. 6 p.829 ~ p.835
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Classification of Native and Cultivated Pyrus Species in Korea by Isozyme Band Patterns
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Abstract
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Of enzymes examined from 50 taxa, only PER and GPI isozymes of adult leaves were useful for taxon identification and classification. Five zymotypes were observed from leaf GOT isozymes and almost all taxa had either zymotype D or E. Nineteen zymotypes were detected from leaf PER isozymes and zymotype E and Q were specific in Korean and Japanese taxa, respectively. Except for Okusankichi, the band at Rf 0.72 was specific only for Chinese taxa and their hybrids, such as Hori and Hattatsu. Therefore, it seemed that the band was conserved only in Chinese taxa. PER isozyme band patterns of young fruit skin and shoot bark were simpler than those of leaf PER. Eleven zymotypes were detected from young fruit skin and eight zymotypes from shoot bark. Twenty one zymotypes were observed from leaf GPI isozymes. Zymotype J and L were frequently observed in Korean taxa, while zymotype Q in Japanese ones. The isozyme analysis and UPGMA cluster analysis using isozyme band patterns were useful tools for taxon identification and the understanding rough taxonomic relationships.
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KEYWORD
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